ANTI-D (RH1) TOTEM is used for blood grouping. It enables the determination of the presence of erythrocytic antigen D on the human red blood cells surface. The reagents are prepared from monoclonal antibodies in a storage medium. The monoclonal antibodies, IgM and IgG type, produced by DIAGAST are derived from the supernatants of in vitro cultures of hybridomas of human originQuantity: 5 x 10 mlClones:• P3x61• P3x21223B10• P3x290• P3x35
Blood grouping is the determination of the presence or absence of ABO and RH1 (D) antigens.
Blood group determination is performed in 2 steps:
Forward typing, where the presence of antigens on the surface of Red Blood Cells (RBC) is investigated with known antisera (
Reverse typing, where the presence of anti-ABO antibodies in the plasma is investigated with known antigens (commercial RBC)
The agreement between forward and reverse typing is a sine qua non condition for the determination of a blood group.
DIAGAST produces monoclonal antibodies and RBC for the forward and reverse typing, respectively.