Dialyse-Argos Pentra is used by the ABX Pentra 120 hematological analyzer(s) as a hemolysing reagent for the assay of white blood cell count and hemoglobin.
SUMMARY AND PRINCIPLE
A sample volume of a whole blood specimen is aspirated into the analyzer where a portion of it is automatically diluted with Diadifton Argos/Pentra. This dilution of the sample is then introduced into impedance particle analyzer where the red blood cell count (RBC) and the thrombocyte count (PLT) is measured. Another portion of the blood sample is also diluted and a hemoglobin lyse reagent (Dialyse-Argos Pentra) is added for hemoglobin (HGB) photometric and white blood cell (WBC) impedance measurements.A thrid portion of the aspirated blood sample is also diluted with lyse reagent (Diafix EO 5 Diff. P120) and diluent is added at the end of the reaction as a stop reagent. From this aliquot the flow cytometer measures white blood cell count (WBC), lymphocyte count (LYM), monocyte cell count (MONO), eosinophil cell count (EO), neutrophil cell count (NEU). A fourth portion of the aspirated blood sample is lysed with a WBC lysing reagent (Dialyse-Baso 5 Diff) for basophil cell count (BASO) measurement in an impedance detector.