A sample volume of a whole blood specimen is aspirated into the analyzer where a portion of it is automatically diluted with Diaton 5D. A portion of this first dilution is further diluted with Diaton 5D. This second dilution of the sample is then introduced into impedance particle analyzer where the red blood cell count (RBC) and the thrombocyte count (PLT) is measured. To the remainder of the first dilution a lysing reagent (Dialyse-HB 5D) is added for the measurement of hemoglobin (HGB). Consult your specific instrument Operators Manual for additional information with respect to procedures and principles for whole blood hematological
analysis.