Anemia assay kit DZ526A

ferritinironlithium

Ferritin is the iron storage protein of the body with a critical role in iron home-ostasis. The iron-free protein, apoferritin (MW 450 kDa) consists of 24 subu-nits, acidic heavy (H, MW 21.0 kDa) and weakly basic light (L, MW 18.5 kDa) monomers, forming a shell to hold ferric iron as ferrihydrite. The basic isofer-ritins are responsible for long-term iron storage function and are mainly detect-able in the human liver, spleen and bone marrow. Serum ferritin is correlated with the available iron storage in body. Ferritin is also used as a marker for iron overload disorders and iron deficiency anemia. Assay Principle The Diazyme Ferritin Assay is based on a latex enhanced immunoturbidimetric assay. When an antigen-antibody reaction occurs between Ferritin in the sample and anti-Ferritin antibodies conjugated to latex particles, agglutination occurs. This agglutination is detected as an absorbance change (560 nm), with the magnitude of the change being proportional to the quantity of Ferritin in the sample. The actual concentration is then determined from a calibration curve prepared from calibrators of known concentrations. Intended use The Diazyme Ferritin Assay is for the quantitative determination of ferritin in human serum, K2EDTA plasma, and lithium heparin plasma on Hitachi 917 analyzer. For in vitro diagnostic use only.