Specifications
Reactions 8
Validated Samples Mammalian cells, Mammalian tissues, nucleated blood, non-nucleated blood, young plant leaves, insects
Technique Hi-C
Labelling Research Use Only
Application(s) Genome Assembly, chromatin conformation analysis, and structural variation detection
Product Description
The Dovetail™ Hi-C Kit uses a single restriction enzyme (DpnII) for chromatin fragmentation prior to proximity ligation. The kit provides all the reagents necessary for performing the proximity ligation steps on animal, plant and prokaryotic samples prior to NGS library generation. The kit is compatible with the Dovetail™ Library Module for Illumina (Cat # 25004) and 3rd party standard paired-end library preparation kits for Illumina sequencing.
The Dovetail™ Hi-C Kit offers the following key benefits:
Proven Hi-C chemistry well accepted in the genome assembly and chromatin conformation research fields
6 hour protocol with less than 3 hours hands-on time easily adopted by molecular biology and genomics labs
Single source kit containing specialized reagents necessary to perform the proximity ligation portion of the protocol (customer provides formaldehyde, EtOH, RNase/DNase free water and SPRIselect beads).