Research test kit KTR-802

allergyIgEIgG

The Eagle Bioscience’s Anti-IgE Receptor (FcɛR1α) Autoantibody ELISA Kit is intended for the quantitative determination of human anti-human IgE Receptor (FcεR1α) autoantibody levels in human serum or plasma samples. The test may be useful for detecting patients with chronic spontaneous urticaria who have developed autoantibodies (mainly IgG) to the IgE receptor. For research use only and not to be used in diagnostics procedures. Anti-IgE Receptor (FcɛR1α) Autoantibody ELISA Kit Anti-IgE Receptor (FcɛR1α) Autoantibody ELISA Kit was developed and manufactured in the US Size: 1×96 wells Dynamic Range: 0 – 100 U/mL Sensitivity: 0.374 U/mL Species Sample: Human Specimen Collection and Storage Only 10μL of human serum or plasma is required for anti-human IgE receptor antibody measurement in duplicate. In the case of serum, whole blood should be collected and must be allowed to clot for a minimum of 30 minutes at room temperature before the serum is separated by centrifugation (850 – 1500 x g for 10 minutes). The serum should be separated from the clot within three hours of blood collection and transferred to a clean test tube. Serum or plasma samples should be stored at 2 – 8°C if the assay is to be performed within 72 hours. Avoid repeated (more than three times) freezing and thawing of the specimen. Assay Principle This ELISA is designed, developed and produced for the quantitative measurement of human anti-hIgE receptor (FcεR1α) autoantibodies in serum and plasma samples. Assay calibrators, controls and diluted patient samples are directly added to wells of a microplate that is coated with recombinant human IgE receptor protein.