The Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit is a module based Enzyme Immunoassay for the confirmation of positive IgG antibodies against SARS coronavirus 2 (SARS-CoV-2) in the first screening. The Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit determines the specificity of antibodies against the main immunodominant antigens (Spike Glycoprotein 1, Spike Glycoprotein 2, Nucleocapsid) of SARS-CoV-2 in human serum or plasma.
Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit is Developed and Manufactured by Generic Assays
Size: 96 wells (24 samples x 4)
Sample Type: Serum or Plasma
Number Of Samples Per Kit: 24 (22 samples + controls)
Not for use in diagnostic procedures.
Assay Principle
Anti-SARS-CoV-2 (S1, S2, N) IgG ELISA Assay Kit is a reagent kit for the determination of IgG antibodies against immunodominant major antigens of the SARS coronavirus-2. The test kit consists of modules separately coated with the major antigens of the virus:
The samples are pipetted horizontally over the 4 strips of a module (e.g. A1+A2+A3+A4), any antibodies present react with the specific antigens bound to the solid phase in the first reaction step. Unbound sample components are removed by a wash step after 45 minutes incubation at 37 °C.
The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 45 min at 37 °C, excessive conjugate is separated from the solid-phase immune complexes by the following wash step.
HRP converts the added colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) into a blue product.