Research assay kit VD331-H100

vitamin Dphosphateserum

The 25-OH Vitamin D3 HPLC Assay kit is intended for the quantitative determination of 25-OH Vitamin D3 in plasma and serum. The 25-OH Vitamin D3 HPLC Assay kit is for research use only and not to be used in diagnostic procedures. The 25-OH Vitamin D HPLC Assay is For Research Use Only Size: 100 tests Controls Included Reference Values (Normal): Summer 50-300 nmol/l (20-120 µg/l) Winter 25-150 nmol/l (10-60 µg/l) It is suggested that each laboratory establish its own normal ranges Product Developed and Manufactured in Germany Product Support in the USA Assay Background Vitamin D is one of the most important essential bioregulators of the Ca2+- and phosphate metabolism in higher animals. Together with the peptide hormones parathormone and calcitonin it is responsible to obtain the Ca2+- and phosphate homoeostasis. The most important physiological functions of vitamin D are the stimulation of the intestinal resorption of Ca2+ and phosphate and their incorporation in the bone matrix. D-vitamins or calciferols arise from provitamins by the UV radiation of sunlight catalyzed splitting of the B-ring of the steran backbone. In the skin, formed vitamin D3 is bound to a vitamin D binding-protein in the plasma, transported into the liver and hydroxylated in position 25 to form 25-OH vitamin D3. Another hydroxylation is performed in the kidney to yield 1,25 (OH)2 vitamin D3 and 24,25 (OH)2 vitamin D3. 1,25 (OH)2 vitamin D3 is the metabolic most active form of vitamin D. Nevertheless, more than 95% of 25-OH vitamin D is 25-OH vitamin D3.