Research assay kit 1018-03

The HLA-A*11:01 IVTDFSVIK Class I Tetramer can be used in flow cytometric analysis to characterise and quantify epitope specific CD8+T cells. The biotinylated monomer can be made into tetramers with optional labels. The Eagle Biosciences HLA-A*11:01 (IVTDFSVIK) Class I Tetramer is for research use only and not for diagnostic or therapeutic procedures. Size: 50, 150, or 500 tests Organism: EBV Epitope: EBNA-4 416-424 (IEDB#: 29466) HLA: Recombinant HLA-A*11:01 with a C-terminal biotinylation. Formulations: peptide-HLA-I monomer, biotinylated peptide-HLA-I tetramer with PE or APC label Buffer: Tris/Maleate PBS pH 7 containing 5% Glycerol Assay Background This HLA-A*11:01 IVTDFSVIK Class I Tetramer protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the β2-microglobulin (β2m) light chain subunit of recombinant HLA class I (HLA-I) complexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labelled with PE-conjugated anti-human β2m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.