Research assay kit FBR31-K01

for endocrine diseasescellserum

The FGF-19 ELISA Assay Kit is designed for quantification of human FGF-19 in serum, plasma, and cell culture supernate samples. The Eagle Biosciences FGF-19 ELISA Assay Kit is for research use only and not to be used for diagnostic purposes. Size: 1×96 wells Sensitivity: 31.2pg/mL Standard Range: 31.2pg/mL-2000pg/mL Alternative Names: Fibroblast growth factor 19 Assay Principle The FGF-19 assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a rabbit polyclonal antibody specific for human FGF-19. Standards and samples are pipetted into the wells and any human FGF-19 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human FGF-19 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and color develops in proportion to the amount of human FGF-19 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human FGF-19, the unknown sample concentration can be interpolated from a reference curve included in each assay. Assay Background Fibroblast growth factor 19 (FGF-19) is a member of a subfamily of FGFs that includes FGF-21 and FGF-23, each member functions as an important regular of nutrient metabolism. The primary source of endocrine FGF-19 is the ileum, bile acids release into the intestine after a meal to induce expression of FGF-19.