Research assay kit IS-I-1400

for immunodetectionplasmaELISA

The L-Lysine ELISA Assay Kit is a competitive ELISA kit for the quantitative measurement of L-Lysine (Lys) in plasma samples. The L-Lysine ELISA Assay Kit is For research use only. Size: 1×96 wells Sensitivity: 2.2 µg/mL Standard Range: 7.42 – 118.72 µg/mL Incubation Time: Overnight Controls Included Assay Principle The L-Lysine ELISA Assay Kit (Enzyme Immunoassay) is for the quantitative determination of L-Lysine (Lys) in plasma samples. After extraction and derivatization L-Lysine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of L-Lysine in unknown samples is achieved by comparing their absorbance with a reference curve prepared with known L-Lysine standards. Storage and Stability The date is indicated on the L-Lysine ELISA kit labels. Once opened the reagents are stable for 1 month when stored at 2 – 8 °C. Once the L-Lysine resealable pouch has been opened, care should be taken to close it tightly with desiccant again. Assay Background L-Lysine (Lys) is an essential amino acid since it’s not produced in the body and must be supplied through the diet. It’s one of the building blocks for protein, peptide and non-peptide synthesis