Research assay kit KBVH015-55

COVID-19SARS-COV-2serum

The Mouse Anti-SARS-CoV-2 (Covid-19) IgG2b Antibody to Whole Spike Protein Quantitative TIitration ELISA kit is used as an analytical tool for quantitative estimation of Anti-SARS-CoV-2 (2019-nCoV) whole spike protein IgG2b antibodies in Mouse serum. The Mouse Anti-SARS-CoV-2 (Covid-19) IgG2b Antibody to Whole Spike Protein Quantitative Titration ELISA is for research use only and not to be used for diagnostic procedures. Size: 1×96 wells Standard Range: 0 ng/ml – 1000 ng/ml Sample Type: Mouse Serum Reactivity: Mouse, Spike Protein Detection Method: Colorimetric, Absorbance read at 450nm Assay Principle The method employs indirect sandwich ELISA technique. SARS-CoV-2 whole spike protein is pre-coated onto microwells. Samples and standards are pipetted into microwells and Antibodies to SARS-CoV-2 (2019-nCoV) whole spike protein present in the sample are bound by the protein antigen. After incubation the wells are washed and followed by addition of HRP-conjugated Detection IgG2b Antibody into each well and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Mouse Anti-SARS-CoV-2 (2019-nCoV) whole spike protein IgG2b in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm. Sample Preparation and Storage Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation. Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Repeated freezing and thawing should be avoided.