Serum reagent FM

for researchlyophilizedphosphate

The Eagle Bioscience’s Human IGF-II (Receptor Grade) is for research use only and not to be used for diagnostic procedures. Product Specifications Source: IGF-II is produced in E.coli using a patented expression system. Description: Human insulin-like growth factor-II (IGF-II) is a single chain 67 amino acid polypeptide that shares structural homology with insulin and human IGF-I. IGF-II plays a critical role in embryonic and foetal development via autocrine, paracrine and endocrine mechanisms. It is a mitogen and also has effects on cell migration and differentiation. IGF-II binds to the type I IGF receptor and the IGF-II/cation-independent mannose-6-phosphate receptor. Its actions are probably mediated through inducing signal transduction via one or both of these receptors. IGF-II also binds to each of the six IGF binding proteins (IGFBPs). This binding also regulates the biological function of IGF-II. IGF-II is highly conserved across species which suggests an important role for the peptide. Human IGF-II is present in high concentrations in normal adult human serum, although the function of this is unknown. IGF-II is also implicated in the pathophysiology of cancer, diabetes and obesity. Purity: >95 % (as determined by HPLC analysis) Quantity: 1 mg Molecular Weight: 7469 ± 2 Daltons – confirmed by Mass Spectrometry N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence Biological Activity: Proliferation of Chinese hamster ovary (CHO) cells (EC50 ≤ 150 ng/ml) Endotoxin (LAL): <0.1 EU/ug Storage/Stability: At least 2 years Appearance: White Powder State: Dried from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25kPa)