The Eagle Biosciences Intact Brentuximab Vedotin ELISA Kit is designed, developed, and produced for the quantitative measurement of Brentuximab Vedotin in serum or plasma. The Intact Brentuximab Vedotin ELISA Kit is for research use only and not to be used for diagnostic procedures.
Intact Brentuximab Vedotin ELISA Assay Kit was developed and manufactured in the US
Size: 1×96 wells
Dynamic Range: 0 – 390 ng/mL
Sensitivity: 0.112 ng/mL
Specimen Collection and Storage
Serum or EDTA-plasma samples are suitable specimens for MMAE- ADC measurement.
Only 10 µL of samples are required for a duplicate determination of MMAE-ADC with this test kit.
Samples should be collected by standard technologies of clinical laboratory practice and recommended by the manufacturer of the sample collection tubes.
Carefully separate the plasma from blood cells to avoid hemolyzation
Samples should be transferred to a clean test tube right after centrifugation and should be stored at 2 – 8C if the assay is to be performed within 72 hours.
Avoid more than three times freeze-thaw cycles of the specimen. Do not use hemolyzed, hyperlipermic, heat-treated or any contaminated specimens.
Assay Principle
The Eagle Biosciences Intact Brentuximab Vedotin ELISA Kit is designed, developed and produced for the quantitative measurement of Brentuximab Vedotin in serum or plasma. The assay utilizes a sandwich immunoassay technique with an antibody that binds to MMAE. Briefly, a mouse monoclonal antibody specific to MMAE is coated onto a microtiter plate. In the assay system, the assay calibrators, controls and test specimen are added to this microtiter plate.