Research assay kit IS-I-0400

cellserumplasma

The L-Arginine ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of L-Arginine (Arg) in serum, plasma or cell culture samples. The L-Arginine ELISA Assay Kit is for research use only and not for use in diagnostic procedures. L-Arginine ELISA Assay Kit Developed and Manufactured in France by ImmuSmol Size: 1×96 wells Sensitivity: 2.1 µM Standard Range: 5.12-200 µM Incubation Time: Overnight Alternative Names: Arginine ELISA, L-Arg, Controls Included Background Enzyme Immunoassay for the quantitative determination of L-Arginine (Arg) in plasma samples. After extraction and derivatization Arginine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards. Specificity: No significant cross-reactivity was observed with L-Homoarginine, Agmatine, L-Citrulline, L-Ornithine, ADMA or SDMA Assay Principle The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate.