Research assay kit IS-I-1300R

plasmaELISAenzyme immunoassay

The L-Histidine ELISA Assay Kit is a competitive ELISA kit for the quantitative measurement of L-Histidine (His) in plasma samples. For research use only and not to be used for diagnostic procedures. L-Histidine ELISA Assay Kit Developed and Manufactured in France by ImmuSmol Size: 1×96 wells Sensitivity: 1.4µM (0.3 µg/mL) Standard Range: 5.3-390µM (1.1 – 81.8µg/mL) Incubation Time: Overnight Reactivity: Reacts with all species Storage: Store at 2-8 °C for up to 6 months Do not use for diagnostic Controls Included Specificity: No significant cross-reactivity was observed with Histamine dihydrochloride, L-Glutamic acid, 3-Methyl-L-histidine & L-Histidinol dihydrochloride Sample Collection and Storage: EDTA Plasma. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) Assay Principle Enzyme Immunoassay for the quantitative determination of L-Histidine (His) in plasma samples. After extraction and derivatization L-Histidine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.