Solution reagent kit BrightBox™

NGSlead

A quick and simple method to quantitate NGS libraries in just 5 minutes! BrightBox eliminates lengthy qPCR dilutions and yields accurate quantitation in a fraction of the time. Library quantitation can be a lengthy, costly, and inaccurate process where variability can lead to unequal library loading and waste time and money. The BrightBox Assay is a quick and simple method to accurately quantitate any NGS library. Faster than current workflows. Preparation and incubation times for the BrightBox assay take a fraction of the time required for other methods. Aliquot Assay Mix • Add Libraries Incubate and read - 5 minutes! • Mix Library with Dve • Load one sample at a time onto Qubit • Estimate Mass - 1 hour • Dilute Library • Prime • Run on instrument -1 hour • Dilute Libraries - 1 hour • Thaw Master Mix • Assemble Master Mix • Aliquot Master Mix • Add Libraries • Cycle and read - 1 hour Why Use BrightBox ✔ FAST Save time with reactions done in just 5 minutes. Assemble reactions quickly with reagents stored at 4 degrees ✔ EASY Quantitate any NGS library made for Illumina sequencers Only detects sequenceable fragments which have a P5 and a P7 adapter Works on any qPCR instrument or plate reader ✔ ACCURATE Reduce manual errors and save time with a premade single Assay Mix Use the five prediluted standards and negative control included in the kit ✔ FLEXIBLE Improve data with consistent pooling based on actual molarity and a single method for quantitation Linear across a large dynamic range