In the presence of peroxidase, hydrogen peroxide reacts with the fluorescent probe, and the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm is proportional to the hydrogen peroxide concentration.
Performance characteristics
Sensitivity - 0.02 μmol/L
Detection range - 0.02-10 μmol/L
Assay type - Quantitative
Precision - Average inter-assay CV: 3.600%Average intra-assay CV: 1.100%
Other instruments required - Micropipettor, Incubator, Vortex mixer, Centrifuge
Storage - Reagent 5 & Reagent 6, 2-8℃; others, -20℃
Valid period - 12 months
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.02-10 μmol/L).