EliGene® Influenza A/B/Pandemic LC kit is intended for the detection of Influenza A and B RNA together with Pandemic variant A/H1N1.
Principle of the method
This diagnostic kit is based on Reverse Transcription-quantitative PCR method in one tube. In this kit primers and labeled probes (FAM, HEX and Cy5) for the detection of Influenza A, B, A/H1N1 RNA and for the detection of internal control are used.
Introduction
Influenza is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The most common symptoms are chills, fever, runny nose, sore throat, muscle pains, headache, coughing, weakness and general discomfort. Approximately 33% of people with influenza are asymptomatic. Flu can occasionally lead to pneumonia, either direct viral pneumonia or secondary bacterial pneumonia, even for persons who are usually very healthy. Typically, influenza is transmitted through the air by coughs or sneezes, creating aerosols containing the virus. Influenza can also be transmitted by direct contact with bird droppings or nasal secretions, or through contact with contaminated surfaces. Influenza viruses can be inactivated by sunlight, disinfectants and detergents. Often, new influenza strains appear when an existing flu virus spreads to humans from another animal species, or when an existing human strain picks up new genes from a virus that usually infects birds or pigs.An avian strain named H5N1 raised the concern of a new Pandemic influenza after it emerged in Asia in the 1990s, but it has not evolved to a form that spreads easily between people.