EliGene® Mycoplasma pneumoniae UNI is intended for DNA diagnostics of pathogenic species Mycoplasma pneumoniae from isolated DNA sample.
Principle of the method
This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit for the detection of Mycoplasma pneumoniae and internal control primers and Molecular Beacons labeled probes (FAM and HEX) are used.
Compatible instruments
The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.
Introduction
Mycoplasma pneumoniae belonging to class Mollicutes has one of the smallest known genomes. It lacks a peptidoglycan cell wall and thus it is resistant to the effects of penicillins and other beta-lactam antibiotics. In humans it causes atypical pneumonia. M. pneumoniae infects the upper and lower respiratory tracts, leading to upper respiratory tract infection, bronchiolitis, tracheobronchitis, bronchitis and community-acquired pneumonia. Infections of the lower respiratory tract generally manifest with a cough, sometimes with dyspnoea, adenopathy, wheezing and, rarely, with respiratory failure. Although M. pneumoniae infections are usually mild, and many are asymptomatic, they are not always self-limiting. M. pneumoniae causes up to 40% of cases of community-acquired pneumonia, but M. pneumoniae respiratory tract infections are also associated with a wide range of extra-pulmonary manifestations, including neurological, cardiac, dermatological, musculoskeletal, haematological and gastrointestinal symptoms.