EliGene® Ureaplasma UNI kit is intended for DNA diagnostics of pathogenic species Ureaplasma urealyticum and Ureaplasma parvum from isolated DNA sample.
Principle of the method
This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit primers and Molecular Beacons labeled probes (FAM and HEX) for the detection of Ureaplasma urealyticum and Ureaplasma parvum DNA and internal control are used.
Introduction
Ureaplasma species colonize the lower urogenital tract in many healthy persons, yet, they can also cause urethritis, endometritis, chorioamnionitis, spontaneous abortion, arthritis and urinary calculi in susceptible adults, as well as prematurity/low birth weight, bacteraemia and meningitis.
Ureaplasma urealyticum is bacterium belonging to the family Mycoplasmataceae. U. urealyticum consists of 14 serovars that can be divided into two biovars, biovars 1 and biovars 2, on the basis of genotypic characteristics. Biovar 1 is made up of four serovars (serovars 1, 3, 6 and 14), whereas biovar 2 comprises ten serovars (serovars 2, 4, 5 and 7–13). Evidence has been presented that the species currently known as U. urealyticum should be separated into two species, namely, U. parvum (previously, U. urealyticum biovar 1) and U. urealyticum (previously, U. urealyticum biovar 2). The majority of human Ureaplasma isolates belong to the new species U. parvum. U. urealyticum (biovar 2) is isolated less often, but is not uncommon.
Ureaplasma sp. is the main cause of non-gonococcal, non-chlamydial urethritis, acute prostatitis and acquired arthritis in men.