John Cunningham Virus (JCV) is a widespread, asymptomatic polyomavirus sharing high homology with BK virus and establishing latency in the kidney. JCV reactivation, after hematopoietic stem cell or solid organ transplantation, is associated with numerous infections of the central nervous system. JCV viral load testing is important to monitor and prevent such complications in transplant patients. JCV ELITe MGB® Kit is a real-time PCR assay designed for the detection and quantification of JC polyomavirus DNA. The assay is CE-IVD validated on a large range of sample types, in combination with ELITe InGenius®, a fully automated sample-to-result solution.
Benefits
Conserved targets
Region of John Cunningham Virus large T antigen gene and an artificial DNA sequence as an internal control
Easy-to-use
Monoreagent, ready-to-use
Best-in class PCR technology
ELITe MGB probes designed with Minor Groove Binder, Superbases® and Eclipse® Dark Quencher
Integrated workflow
Fully automated process for extraction, amplification and result analysis with ELITe InGenius
Quantitative result
International units (IU/mL) correlated to the 1st WHO International standard for Human JCV for NAAT (NIBSC Code 14/114)
Copies/mL (cp/mL)
Storage of standard curve
60 days
Eluate storage
Additional testing including multiple PCR from the same eluate, retesting or archiving
Complete transplant menu -
Complete transplant pathogen monitoring range