Polystyrene Petri dish CultureCoin®

The Esco Medical CultureCoin® is an IVF culture dish designed specifically for the MIRI® TL. The MIRI® TL is Esco Medical’s time-lapse incubator that captures real-time images of developing embryos which can be viewed as time-lapse videos.The CultureCoin® is a single-use, gamma-sterilized culture dish that has oxygen plasma-treated surfaces for effective prevention of bubble formation. Its ergonomic design makes it easy for embryologists to load a total of 14 embryos while being able to safely and securely handle them inside the incubator. This culture well features a 300µm, optically clear area for embryo placement which is optimized for microscopy. Technical Specifications Overall dimensions ⌀ 71 x 10 mm Weight empty 0.5 gram Material Polystyrene Temperature range 25 ‐ 40 °C CO2 range 1.9 ‐ 10% O2 range 2.0 ‐ 4.9% Sterilization method Gamma beam Lifetime 2 years Toxicity tested Embryo toxicity tested with thawed 1 cell mouse embryos. Expanded Blastocyst rate on the culture dish after 96 hours > 80 % (n=150)0.6 bar (8.70 PSI) How to use the CultureCoin® Unpack the IVF culture dish inside a workstation or laminar flow hood to provide an aseptic environment. Leave the dishes inside the workstation with lids on for off-gassing for 12 hours. Pre-warm the dishes on a heated stage or inside a CO2 incubator prior to filling with pre-equilibrated media. Start filling all culture wells with approximately 25µL of pre-equilibrated IVF culture media in the workstation. The user also has an option to fill the wash wells with approximately 23µL of culture media.