Enzyme reagent N - GARDE

reagent mediumdyefor PCR

Mycoplasma detection by the direct culture procedure is time-consuming and some mycoplasma species are difficult to cultivate. With mycoplasma PCR testing, results are obtained within a few hours, since the presence of contaminant mycoplasma can be easily detected simply by verifying the bands of amplified DNA fragments in electrophoresis. There is no need to prepare probes labeled with radioisotopes, or to calculate enzyme, dNTP’s or buffer concentrations. Instead, a ready-to-use, optimized PCR mix is supplied. The reaction mix in the mycoplasma detection kit contains a precipitant for direct loading of PCR products onto agarose gel. Various Spices of Mycoplasma Detection The primer set allows detection of various mycoplasma species (M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. bovis, M. pneumoniae, M. pirum and M. capricolum), as well as Acholeplasma and Spiroplasma species, with high sensitivity and specificity. N-GARDE Mycoplasma PCR Reagent set is based on a simple assay protocol and has the following advantages: Highly sensitive Mycoplasma-specefic primers with broad range Convenient and user-friendly: supplied with complete reaction mix (with Taq polymerase). Requires no more than 10-20 minutes of actual work Samples are easy to prepare Results are easily determined with a single PCR process Rapid: results obtained in no more than 5 hours No need for internal control application steps No loading dye needed for the agarose gel