Iscove's Modified Dulbecco's Medium is a modification of DME using high glucose (4500 mg/l).It contains sodium pyruvate and additional amino acids ,HEPES buffer, selenium and other components. IMDM was originally designed for the serum-free growth of primary hematopoietic cells when, properly supplemented. When used in conjunction with serum ,IMDM supports the growth of a broad spectrum of mammalian cells. IMDM is ideal for rapidly proliferating high density cell cultures in a 5% CO2 atmosphere.