Dye-labeled calibration oligos are 5’ fluorescent 10-mers dT oligonucleotides used as a reference to calibrate real-time qPCR thermocyclers.
Calibration is a preliminary step meant to adjust spectral profile for each single dye on the qPCR platforms. This allows to compensate for crosstalks between dyes (channels) in the context of multiplexed assays, which is critical for appropriate result analysis.
Please contact our scientific support for additional options
Specifications
Packaging
Kits components -
1 x 2 mL tube containing 5 nmol of calibrating oligo
Chemistry
Sequence one letter code -
5' HiLyte™ Fluor 647 - TTT-TTT-TTT-T 3'
Linkage -
Phosphodiester
Molecular Mass/ Weight -
4372.7
Properties
Absorbance (nm) -
650
Emission (nm) -
675
Color -
Red
Modification
Modification Position -
5'
Conjugation type -
Fluorescent dyes
Modification Name -
HiLyte™ Fluor 647
Conjugation -
Conjugated