Dye reagent JOE-T10

for researchcalibrationlyophilized

Dye-labeled calibration oligos are 5’ fluorescent 10-mers dT oligonucleotides used as a reference to calibrate real-time qPCR thermocyclers. Calibration is a preliminary step meant to adjust spectral profile for each single dye on the qPCR platforms. This allows to compensate for crosstalks between dyes (channels) in the context of multiplexed assays, which is critical for appropriate result analysis. Specifications Packaging Kits components - 1 x 2 mL tube containing 5 nmol of calibrating oligo Chemistry Sequence one letter code - 5' JOE - TTT-TTT-TTT-T 3' Linkage - Phosphodiester Molecular Mass/ Weight - 3647.5 Properties Absorbance (nm) - 529 Emission (nm) - 555 Color - Yellow Modification Modification Position - 5' Conjugation type - Fluorescent dyes Conjugation - Conjugated Quantity & Purity Purification Method - RP-HPLC Resuspension condition - To make up to 100 µM concentration, add 50 µl of recommended buffer. To make up to 20 µM concentration, add 250 µl of recommended buffer. To make up to 5 µM concentration, add 1000 µl of recommended buffer. Storage Buffer - TE buffer (pH 8) or dH2O