Enzyme reagent kit Mu-MLV

reverse transcriptasefor researchliquid

Mu-MLV (Moloney Murine Leukemia Virus) generates complementary DNA strand starting preferably from single-stranded RNA in the presence of a primer, or from RNA-DNA hybrid. Mu-MLV reverse transcriptase has lower RNase H activity than AMV reverse transcriptase, which is an advantage when synthesizing cDNAs from long mRNAs. Mu-MLV lacks the 3’ ’ 5’ exonuclease activity. It is recommended to use 10 U/µg RNA for optimum reverse transcription. Applications This enzyme is particularly suited for long cDNA fragments in the context e.g. of generating cDNA libraries or for first strand cDNA synthesis prior to RT-PCR. Specifications Packaging Kits components - One tube (200 µL) of Mu-MLV reverse transcriptase 40000 units (200U/µl) 5 Tubes of cDNA buffer (clear cap) 250 mM Tris-HCl (pH 8.3), 375 mM KCl, 50 mM DTT, 15 mM MgCl2 Quantity & Purity Concentration - 200 U/µ Activity Template type - RNA Unit definition - One unit is the amount of enzyme that incorporates 1 nmole of dTTP into acid - insoluble form in 10 minutes at 37 °C using polyA-oligodT 12-18 as substrate.