Medium reagent BA-ME010-004

for researchprotein

2’-O-(2-Methoxyethyl)- oligoribonucleotides or 2’-O-MOE have an analogue chemical structure to RNA excepted that a methoxy-ethyl residue is attached at the 2’-O-position. The chemical group at this position confers to the oligo backbone a highest nuclease resistance and a better binding affinity compared to the classical RNA molecule making it a useful tool for antisens applications. Benefits - Nuclease resistant - High binding affinity - Reduced unspecific protein binding - Superior half life in tissues Phosphorothioate bonds possess an increased resistance against nucleases due to the substitution of a non-bridging oxygen by sulphur. Length: From 5 to 139 bases Synthesis scale: 10 nmol - 40 nmol - 200 nmol - 1000nmol - 2.5 µmol - 5µmol - 10 µmol (Larger quantities available on request) Modifications: More than 300 modifications available Purifications: SePOP desalting, HPLC, PAGE, Dual HPLC, UltraPureGold™ Quality Control: MALDI-TOF MS Format: Dried. other formats on option Packaging: 2 mL tube, 96-well or 384-well plates Specifications Chemistry Linkage - Phosphorothioate Linkage Modification Modification Position - 3' 5' IN Storage & stability Resuspension condition - - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds