Solution reagent kit FG-0510-48

magnetic bead-basedproteinase Kfor DNA extraction

- Applicable to difficult-to-lyse microbial samples, such as Gram-positive bacteria, Mycobacterium tuberculosis, yeast, fungi, spores, etc. - The resulting DNA has high molecular weight, high integrity, and no small fragments with shearing - The crushing efficiency is above 90%, which effectively avoids false negative results. - High yield, no polymer DNA contamination - The extraction process is compatible with different types of wall-breaking enzymes Product Overview Description This kit utilized FireGene's original magnetic bead purification technology and a unique buffer system, which combines physical-mechanical and enzymatic digestion methods to fully lyse bacterial cell walls, especially for Gram-positive bacteria and other difficult-to-lyse bacteria. A large amount of nucleic acid is released, which doubles the yield of nucleic acid extraction, effectively avoids false negative results caused by insufficient wall breaking, and is suitable for extracting total DNA from various microorganisms. The extraction process does not involve chloroform, phenol and other reagents. The extracted genomic DNA has large fragments, high purity, stable and reliable quality, and can be used for downstream experiments such as enzyme digestion, library construction, cloning, PCR, quantitative PCR, chip detection, and high-throughput sequencing. The kit can also be efficiently used with automated liquid workstations and magnetic bar method magnetic bead automatic extraction systems for fast, high-throughput extraction. Specifications Applications - Enzyme digestion, library construction, cloning, PCR, quantitative PCR, chip detection, and high-throughput sequencing