DNA extraction assay kit PreCursor-M AnoGYN

actinreal-timemolecular

PreCursor-M AnoGYN is a methylation-specific molecular assay to detect promoter hypermethylation of the human genes ASCL1 and ZNF582 in bisulfite-converted DNA prepared from human samples, for instance formalin-fixed paraffin embedded anal biopsies or anal swab specimens. Special Features: One-step PCR: Multiplex format enabling simultaneous amplification and detection of the two targets and controls in a single reaction Ready-to-use mastermix: Just add bisulfite-converted DNA Reliable: Robust Good reproducibility Built-in check for sample quality and normalization. Details Method: Multiplex real-time methylation-specific PCR assay Target: Human genes ASCL1 and ZNF582 Built-in reference and sample quality control: β-actin (ACTB) Calibrator: Contains a standard concentration of low-copy DNA fragments of the two targets ASCL1, ZNF582 and the control gene ACTB – also used for run validation Sample types: Bisulfite-converted DNA prepared from human samples, for instance formalin-fixed paraffin embedded anal biopsies or anal swab specimens Sample volume: 5 μL of bisulfite-converted DNA Total time to result: Ranging between 2 to 24 hours depending on the level of automation (incl. DNA extraction and bisulfite conversion) Equipment: Mic qPCR cycler (Bio Molecular Systems), Rotor-Gene Q 5plex system (Qiagen) or ViiA 7 qPCR system (Thermo Fisher Scientific)