Phosphate buffer reagent PE LB™

enzymeproteasechromatography

Bacterial PE LB™ has been developed for the extraction of soluble proteins and inclusion bodies from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. Bacterial PE LB™ lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal force. This kit is provided with an optional protocol for the formation of spheroplast and removal of lytic enzyme (Lysozyme) prior to lysis and extraction of the bacterial proteins. Bacterial PE LB™ is based on organic buffering agents and utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as reducing agents, chelating agent, and protease inhibitors cocktail may be added into Bacterial PE LB™. This reagent has been tested for use with several widely used bacteria including E. coli strains. Bacterial PE LB™ is compatible with most downstream applications including running various chromatography, gel electrophoresis applications, and protein folding procedures. Bacterial PE LB™ is also compatible for protein estimation with NI™ protein assay (Non-Interfering Protein Assay)™. For bacterial lysis, the kits are suitable for extracting soluble proteins from approximately 30g wet cell pellets for every 100ml Bacterial PE LB™. When the kits are used for extracting soluble proteins from spheroplasts, they are suitable for approximately 9g wet cell pellets for every 25ml Bacterial Suspension Buffer supplied.