Assayed reagent CB-X™

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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major problem for researchers is to select a protein assay from the vast selection on the market that is compatible with their protein sample. CB-X™ Protein Assay eliminates this problem as it is designed to be compatible with all commonly used buffers and conditions in protein isolation, storage and assays. For protein samples in simple, uncomplicated, aqueous buffers, CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. CB-X™ Protein Assay uses a protein dye that is an improvement on the Bradford Coomassie dye reagent (Figure 1) For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation (figure 1). These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents (Table 1). The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay. Figure 2 demonstrates the effect of CB-X™ in the presence of 1% Triton® X-100 or 1% SDS. If the protein sample does not contain interfering agents, then a straightforward, single reagent assay is performed to give a linear response.