Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.
Eliminates worries about urea induced protein carbamylation or waste of unused reagents, as fresh and ready-to-use reagents are prepared every time.
The Equilibration Buffer is supplied with a proprietary buffer necessary to support efficient reduction of the disulfide bridges and alkylation of the thiols, while minimizing reoxidation of the competing thiol pairs in protein samples. Improves resolution and prevents streaking of protein spots on 2D gels.
Features
Dry urea based premixed and ready-to-use equilibration buffers.
Fresh and ready to use reagents every time.
Prevents urea induced protein carbamylation or waste of unused reagents.
Improves resolution and prevents streaking of protein spots on 2D gels.
Applications
For equilibration of IPG buffer strips before running second dimension electrophoresis.