Molecular biology microplate AutoScreen

96-well

AutoScreen 96-Well Plate is used for purification of sequencing samples reactions prior to analysis on capillary array electrophoresis (CAE) sequencers such as MegaBACE 1000 or ABI Prism Sequencing instruments. Includes DNA Grade Sephadex G-50 rehydrated in double-distilled water as well as 0.05% Kathon™ CG/ICP Biocide added as a preservative. Preferred loading volume is 15 to 20 µL. The suggested range for loading volumes to achieve optimum purification is 10 to 20 µL. A plate lid may be used during this centrifugation step to improve consistency of volume recovery and reduce the likelihood of contamination during purification steps in sample sequencing. AutoScreen 96-Well Plates used with MegaBACE 1000 can show two to three times lower signal intensities compared with intensities from Ethanol-Precipitated reactions. This does not affect read lengths. AutoScreen 96 is also applicable to other size exclusion chromatography applications.