Protein ladder PiNK Plus

for Western blot

The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kilodalton (kDa). The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), estimate molecular weights of proteins of interest, and evaluate western transfer efficiency. Features Broad range: 10-175 kDa (Tris-glycine-SDS running buffer) Ready-to-use: supplied in a loading buffer for direct loading on gels Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with a green and a red dye Sharp bands Applications Monitoring of protein migration during SDS-PAGE. Monitoring of protein transfer onto membranes during Western blots. Sizing of proteins on SDS-PAGE gels and Western blots. Storage Buffer Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 4.8 M Urea, and 12 % (v/v) Glycerol. Quality Control The quality of the PiNK Plus Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality. PiNK Plus Prestained Protein Ladder Protocol 1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil. 2. Mix thoroughly to ensure the solution is homogeneous.