Stain reagent ECLong

for Western blotfor electrophoresischemiluminescence

The principle of ECLong is based on chemiluminescence and is very convenient to detect the Horseradish peroxidase (HRP) activity in many assays such as Western blotting, Southern blotting, and Northern blotting. HRP catalyzes the chemiluminescent oxidation of cyclic diacylhydrazides such as luminol by hydrogen peroxide (H₂O₂). ECLong can enhance the luminol-dependent chemiluminescence and be wildly used to detect the present of HRP-conjugated antibodies or streptavidin which binding to antigen or nucleotide sequence respectively. Application Western blotting Quality Control The quality of the ECLong is tested on a lot-to-lot basis to ensure consistent product quality. Size: 250mL x 2 Features Signal Duration: 12 hours Detection Method: X-ray film or imaging acquisition system Suggested Antibody Dilution: Primary: 1/1,000 – 1/50,000 Secondary: 1/50,000 – 1/250,000 Lower Detection Limit: Low-Picogram (10⁻¹⁴) High-Zeptomole (10⁻¹⁹)