Dye reagent GT500

for capillary electrophoresis

GT500 Size Standard is developed to use as an internal size standard with GT-5 (5 dye system) and GT-6 (6 dye system) dye systems in Applied Biosystem™ capillary electrophoresis platforms. Use of this internal lane size standard provide ease of data analysis and precision in sizing DNA fragments. GT500 Size Standard consists of 27 fragments in size range from 60bp to 500bp where 23 fragments are spaced 20bp apart and 4 fragments with intermediate space. These fragments are of size: 60, 80, 100, 113, 120, 140, 160, 180, 200, 220, 230, 240, 260, 280, 300, 320, 330, 340, 360, 380, 400, 420, 430, 440, 460, 480, and 500 nucleotides. Features GT500 Size Standard fragments generate an optimum sizing curve Used for various fragment analysis application such as microsatellites, fragment-length polymorphisms etc. Each fragment of the GT500 is double stranded DNA labelled with an orange fluorescent dye which has its excitation in the range of 610-645 nm. Capillary electrophoresis produces single peak with denatured condition.