The examined protein, diffusing in agarose gel containing a specific antibody will form an immuno-complex, visible as a ring around the well. The ring diameter is direct proportional to the concentration of the analysed protein. The proportion corresponds to the diffusion time. In fact, at the end (96h), the square of diameter will be in linear proportion to the concentration of the sample.
With the plate is supplied a reference table in which each diameter of the halo is associated a concentration.
SAMPLES
Serum, plasma. Stability 6 days at 4°C.
REAGENTS
Plate:
Agarose gel containing the goat antiserum IgM