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  3. Enzyme reagent
  4. Guangdong Ardent BioMed Co., Ltd

Enzyme reagent kit
for PCRdiagnosticfor nucleic acids

enzyme reagent kit
enzyme reagent kit
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Characteristics

Type
enzyme
Applications
diagnostic, for PCR, for nucleic acids
Micro-organism
coronavirus
Storage temperature

-20 °C
(-4 °F)

Description

This kit is an in vitro diagnostic test used for the detection of a new type of coronavirus (COVID19). Samples can be obtained from throat swab or sputum. Reaction Mix is basic components of this detection kit. The reacton mixes in this kit contain specific primers and probesfor N gene andORF1ab of COVID-19, and RNase P gene assample internal control (IC), as well as all components needed for efficient RT-PCR reactions Positive control isthe mix ofsingle-stranded RNA ofspecific regions of novel coronavirus, required to be dispensed into 200 μL PCR tubes with 5μL per reaction according to the required number of reactions. Please avoid repeated frozen-thawed cycles to avoid the degradation of RNA templates Components from different batch number kits is not recommended to be used interchangeably 1. All reagents should be stored at -20℃ unyil the expiration date listed on the outer kit box. 2. Protect Reaction Mix from light during storage. 3. Repeated freezing and thawing (more than five times) of reagents should be avoided. 4. Expiration date: 9 months 1. Biological cabinet. 2. Appropriate real time PCR instrument: ABI7500 、 ABI QuantStudio 6/7/12K; Roche Lightcycler®480/1536/Nano; Agilent Mx3000P/3005P; Qiagen Rotor-Gene 6000 / Q; Bio-Rad CFX384/CFX96, Bio-Rad Touch / iQ5; Cepheid Smartcycler/Smartcycler II; Eppendorf Mastercycler. 3. Appropriate nucleic acid extraction system or kit 4. Desktop centrifuge (suitable for 96-well plate or 8-strip tube). 5. Centrifuge with a rotor for 0.2ml reaction tubes or plate. 6. Vortex mixer. 7. Adjustable pipettes (with maximum capacity of 2μL, 10μL, 50μL, 100μL, and 200μL, respectively).

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