Buffer solution reagent P1011 500U

enzymeDNA polymeraseTaq DNA polymerase

Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product. Unit Definition One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate. Storage Buffer 20mM Tris-HCl (pH 8.0), 100mM KCl, 3.2mM MgCl2, 1mM DTT, 0.1% Triton X-100, 0.1% Tween20, 0.2mg/ml BSA, 50% (V/V) glycerol. 10× PCR Buffer without Mg2+ 100mM Tris-HCl (pH 8.8), 500mM KCl, 1% Triton X-100. Applications • PCR amplification of DNA fragments as long as 5 kb • DNA labeling • DNA sequencing • PCR for cloning Payment & Shipping Terms: Minimum Order Quantity: - 1Bag Packaging Details: - small package or bulk distribute or OEM Delivery Time: - 8 work days Payment Terms: - L/C, D/A, D/P, T/T, Western Union, MoneyGram Supply Ability: - 100 Bag/Bags Per Day