Dye reagent DSRed

agarose gelnucleic acidfor nucleic acids

DSRed is a sensitive, stable and non-toxic fluorescent nucleic acid dye for staining nucleic acids (dsDNA, ssDNA, RNA) in agarose gels and polyacrylamide gels. DSRed has a sensitivity higher than EB (Ethyl Bromide). DSRed binds nucleic acid molecules by intercalation, and is nonmutagenic at working concentrations, is safer and more environment-friendly than EB. Safety Description: This product has a special molecular structure and cannot pass through cell membranes or latex gloves. This product has passed biosecurity tests, is nonmutagenic at working concentrations. And it is environmentally safe and can be dumped directly into sewers. Storage Store at room temperature, protected from light. DSRed is stable for at least one year from the date it is received. Staining Protocols Because nucleic acid molecules bind to the dye, it affects their own migration in the electrophoresis process, and also causes the adjacent nucleic acid bands to interact with each other, it is more recommended to use post-staining. For polyacrylamide gels, post-staining is also recommended. In order to adapt to the usage habits of users of precast methods, we have developed a series of LD DNA Markers for the standard DNA molecular weight of macromolecule nucleic acid dyes. Therefore, when users precast agarose gel with noncytotoxic nucleic acid dyes such as DSRed, pair it with the DongSheng Biotech LD DNA Marker series. As shown on the left, the DNA Marker DSTM 5000 in the DSRed pre-stained gel is squeezed by the strips and cannot be effectively separated, while the LD DSTM 5000 bands can be separated normally.