General RNA Extraction Kit provides a simple method of isolating total RNA from a wide range of sample types and amounts. In general, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt which is capable of protecting the RNA from endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed through a spin column containing a clear silica-based membrane where the RNA binds. Any impurities are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-Free Water and is suitable for use in a variety of downstream applications.
Downstream Applications
Purified RNA is free of impurities and enzyme inhibitors, and have an A260/280=1.8-2.0, is suitable for applications such as:
• Reverse transcription PCR (RT-PCR)
• Real-time quantitative PCR (qPCR)
• Northern blotting
• Nuclease protection assays
• RNA amplification for microarray analysis
• cDNA library preparation after poly(A)+ selection
Features
• Stable yield
• Reliable performance of high-quality purified total RNA in downstream applications
Payment & Shipping Terms:
Minimum Order Quantity: - 1 box
Packaging Details: - small package or bulk distribute or OEM
Delivery Time: - 8 work days
Payment Terms: - L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: - 100 Box/Boxes per Day