Taq DNA polymerase reagent HS

for real-time PCR

HS Probe qPCR Mix with UDG is designed for high-performance, high-throughput real-time PCR using sequence-specific fluorogenic probes. It contains the components (except primers, DNA template and probe) that necessary to perform PCR. The core component of the mix is OptimusTM Hotstart Taq DNA Polymerase, a hot-start polymerase with antibody-like modification, which brings high specificity by reducing non-specific products as the polymerase activity is temperature-dependent and inhibited at room temperature. Combined with well-optimized buffer, it can significantly improve amplification efficiency. This reagent introduced dUTP/UDG system, which can remove the PCR products containing dUTP before PCR reaction, and effectively avoid the influence of cross-contamination of amplification products on the quantification. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc. Applications • Probe gene expression analysis • Probe Low-copy gene detection • Probe microarray validation • Probe gene knockdown validation Features • This kit is suitable for fluorescence quantification by probe method • This kit is compatible with many real-time systems • Hot-start technology brings high specificity and reproducible amplification • dUTP/UDG system, effectively prevent PCR product contamination Composition of the 2× HS Probe qPCR Mix with UDG KCl, MgCl2, dNTP/dUTP Mix, OptimusTM Hotstart Taq DNA Polymerase, UDG (Uracil-DNA Glycosylase) and other optimized buffer components.