Enzyme reagent kit K004-A

dyeDNA ligasefor DNA sequencing

Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines fragmentation, end repair and end “A” tailing into one step, greatly shortening the time of library construction and reducing the error caused by tedious steps. After fragmentation and end repair, the product can be directly ligated with adapter without additional purification, and the subsequent procedure is the same as that of #K001 Fast DNA Library Prep Kit. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration. Payment & Shipping Terms: Minimum Order Quantity: - 1Bag Price: - / Packaging Details: - small package or bulk distribute or OEM Delivery Time: - 8 work days Payment Terms: - L/C, D/A, D/P, T/T, Western Union, MoneyGram Supply Ability: - 100 Bag/Bags Per Day 【Components】 Component - 24 rxns - 96 rxns FEP Buffer - 120 μl - 480 μl FEP Enzyme Mix - 240 μl - 2×480 μl Fast DNA Ligase - 120 μl - 2×240 μl Fast Ligation Buffer - 600 μl - 4×600 μl 2× HIFI Library PCR Master Mix - 600 μl - 4×600 μl Primer Mix* - 120 μl - 480 μl Neutralization Buffer - 120 μl - 480 μl