The RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate.
Applications
--Plasmid and genomic DNA preparation
--Removal of RNA from recombinant protein preparations.
--Ribonuclease protection assays
--Mapping single-base mutations in DNA or RNA
Molecular Weight
13.7 kDa monomer.
Definition of Activity Unit
One unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm when yeast RNA is hydrolyzed at 37°C and pH 5.0.
Fifty units are approximately equivalent to 1 Kunitz unit.
Storage Buffer
The enzyme is supplied in: 50 mM Tris-HCl (pH 7.4) and 50% (v/v) glycerol.
Payment & Shipping Terms:
Minimum Order Quantity: - 1 bag
Packaging Details: - small package or bulk distribute or OEM
Delivery Time: - 8work days
Payment Terms: - L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: - 100 Bag/Bags Per Day