Enzyme reagent C001 series

nucleic acidfor mutation detectionliquid

Magigen CRISPR Cas12a protein is a programmable DNA endonuclease guided by a single guide RNA (gRNA). Cas12 requires only a single crRNA, simple designed. It is more easier to delivered into cells with less volume. Cas 12 provides new target sequence selection with TTTN PAM, suitable for a broad reaction temperature range of 20℃-48℃. Introduction With its pure protein system that resists distraction and low off-target rate, The CRISPR-Cas9 technologies is a safe and common method in the field of gene editing. Unit Definition ♦ This product is a recombinant protein that carries NLS fragments at both the N- and C-terminals. ♦ This product 1pmol=151 ng. ♦ The design and stabilization of sgRNAs have a great influence on the reaction system, and whether sgRNAs can be degraded by gel electrophoresis before the reaction is carried out. Product Features 1. Requires only a single crRNA, easy design. It is more easily delivered into cells with less volume. 2. Provide new target sequence selection with TTTN PAM. 3. a broad reaction temperature range of 20℃-48℃. 4. The affiliated cleavage activity generated after cleavage of the target site can be used in a highly sensitive and specific nucleic acid detection scheme. Product Performances According to tested results below, using different concentrations of Cas12a protein to cut ssDNA, it can be found that a sufficient amount of Cas12a can cut DNA in about 12 minutes.