Enzyme reagent D001 series

DNA polymeraseBst DNA polymerasegenome editing

The Bst DNA Polymerase, Large Fragment, is a DNA polymerase from Bacillus stearothermophilus that is modified so that it retains its 5'→ 3' DNA polymerase activity while lacking the intrinsic 5'→ 3' exonuclease domain. This polymerase also has strand displacement capabilities making it an ideal candidate for isothermal amplification, such as LAMP, Loop-Mediated Isothermal Amplification. Introduction Bst DNA Polymerase (large fragments) is part of the Bacillus stearothermophilus DNA polymerase with 5' →3' DNA polymerase activity, as well as strong strand displacement activity, but 5' →3' exonuclease activity is missing. ● Non-specific amplification control ● High sensitivity ● High purity Unit Definition The amount of enzyme required to incorporate 10 nmol of dNTP into an acid-insoluble precipitate at 65°C for 30 min, defined as 1 active unit (U). Product Performances Comparing Magigen Bio Bst DNA Polymerase (left panel) and Bst DNA Polymerase from supplier N(right panel); a comparative amplification reaction was performed with multiple negative-tests were set, and the experimental time was 90 min. The results show that the Magigen Bst DNA Polymerase has better LAMP perfomences since the positive curve is tall and straight (red line), and the negative curve has no peak (gray line).