Enzyme reagent C007 series

Cas9 ProteinT7 endonuclease Ifor mutation detection

T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double stranded DNA. The cleavage site is the first, second or third phosphodiester bond that is 5´ to the mismatch. Introduction Magigen T7 Endonuclease can be used to detect gene mutation, SNP, TALEN or CRISPR / cas9 mutants, identify mismatched DNA, and resolve four-way cross DNA or branched DNA. Detection of heteroduplex or cleavage of DNA; It is used to randomly cut linear DNA for shot gun cloning, etc. Unit Definition The amount of enzyme required to convert pUC (AT)* of a 90% or more superhelier cross-shaped structure into a linear structure of more than 90% in a 50 μl reaction system at 37°C for 1 hour is defined as one unit of activity. Product Features T7 Endonuclease I is a substrate structure-selective enzyme that exhibits different cleavage activities for different DNA substrates. Therefore, when cutting a specific substrate, it is necessary to explore and control the amount of enzyme and reaction time.