Infectious disease detection kit SMV002

virusmonkeypoxserum

Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR) is used for the detection of nucleic acid from monkeypox virus in serum and human pustular or vesicular rash specimens from individuals suspected of monkeypox virus infectious. By specifically detecting the nucleic acid fragments of monkeypox virus, monkeypox virus can be quickly identified, which is suitable for rapid diagnosis of related diseases caused by monkeypox virus infection. [Test Principle] This kit uses polymerase chain reactor-fluorescent probe technology to design specific primers according to the nucleic acid sequence of monkeypox virus for amplification of corresponding nucleic acid fragments. At the same time, the highly specific probe can be combined with the corresponding nucleic acid fragment and hydrolyzed under the action of exonuclease activity to generate fluorescence signal for detection. Real-time amplification curve can be obtained according to the relationship between fluorescence signal and amplification cycle number. [Materials Required but Not Provided] 1) 48 or 96-well Polypropylene PCR Microplate. Or 0.2 mL Polypropylene PCR Tube Strips with Flat Cap, 2) Nucleic acid extraction reagents or Preservation solution, 3) Pipettes and Pipetting tips (10μL, 200μL and 1000μL tips with filters), 4) Centrifuge, 5) Desktop vortex mixer, 6) Disposable powder-free gloves and surgical gowns [Applicable Instrument] Recommended PCR instruments include: Applied Biosystems™ 7500 real-time PCR system; ABI 7500 Real-time fluorescence quantitative PCR instrument , SLAN®-96S/96P real-time PCR system, Thermofisher QuantStudio™5 Real-time fluorescence quantitative PCR,